Staff Research Associate I - Ophthalmology

Description

Under the direction of the PI, the incumbent is responsible for molecular biology aspects of the research project. Duties include carrying out molecular biology procedures, i.e. cloning, mutagenesis, sequencing, polymerase chain reaction, preparation of recombinant plasmids, and general recombinant DNA methods. Duties also include culturing cells and carrying out biochemical assays. The incumbent will be engaged in research projects involving cloning and characterization of genes. The incumbent will maintain an inventory of cDNA, DNA plasmid stocks, the lab and equipment in order.Qualifications

Required

Knowledge of molecular biology techniques

Skills and extensive experience in human and mouse cell culture technique,

Skills in cryo-section

Skills in isolating and purifying high quality DNA and RNA

Skill in sub-cloning of cell lines

Ability to use molecular biology software

Ability and experience to perform immunohistochemistry

Knowledge of cell culture, including thawing cells, culturing cells and freezing cells

Ability to perform protein analysis by gel electrophoresis and Western Blots

Ability to establish and maintain records and supply stocks

Skill in maintaining the lab and equipment in working condition

Ability to establish and maintain cooperative working relationships with co-workers

Demonstrated experience and skills in using the following Pipettes, Centrifuges,

Balances, Incubators, Thermal cyclers, Dry blocs, Shakers, Autoclaves, Water baths, Liquid nitrogen tanks, DNA synthesizer, PH meter, sequences

Preferred

Ability to work with mice, setting up breeding pairs and recording births and weaning animals

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